Ocean-optics Curie UV-VIS Emission User Manual

The Curie UV-VIS Emission Spectrofluorometer Installation and Operation Manual Document Number 640-11100-000-02-0406 Offices: Ocean Opt

1: Introduction  Software and Technical Resources CD: Each Curie system order comes with Ocean Optics’ Software and Technical Resources CD. This C

1: Introduction 2. Start the OOIBase32 installation process. Installing from CD: a. Insert the Software and Technical Resources CD containing the

1: Introduction Installing Custom Curie Software to Activate Relative Irradiance Mode Caution Do NOT connect the Curie System to your PC until afte

1: Introduction Connecting the Curie to a PC The Curie connects to a PC via a standard USB device cable. USB 1.1 is currently supported. Caution Be

1: Introduction 8 640-11100-000-02-0406

Chapter 2 Configuration Introduction This chapter provides instructions for configuring the OOIBase32 Configure Spectrometer options so that the a

2: Configuration Default Spectrometer Configuration File The Default Spectrometer Configuration File screen prompts you to select a default .SPEC f

2: Configuration 3. In the USB Serial Number field, select the serial number of the USB2000 spectrometer in your Curie system from the drop-down li

2: Configuration 5. On the A/D Interface tab, enter the same values that you entered in the Configure Hardware screen (see Configure Hardware Scre

2: Configuration 3. In the External Trigger Mode field, select Software Trigger to enable the pushbutton. 4. To save files when the pushbutton is

Copyright © 2001-2006 Ocean Optics, Inc. All rights reserved. No part of this publication may be reprodu

2: Configuration Note If you do not enable the Autoincrement Filenames function, a save file dialog box will open every time you push the button (if

2: Configuration 3. In the External Trigger Mode field, select None to disable the pushbutton, and click OK. 4. Push the pushbutton on the Curie.

2: Configuration • Method 2: Check the Strobe/Lamp Enable box on the Acquisition Parameters toolbar. Once you enable the strobe via any of the op

2: Configuration 3. On the External Trigger tab, configure your external triggering mode to activate your pushbutton and data save option, if nece

2: Configuration 5. On the OOIBase32 main screen, check the Strobe/Lamp Enable option. The pulsed xenon lamp activates, and a fluctuating trace ap

Chapter 3 Using the Curie System Introduction This chapter provides you with instructions for performing experiments using the Curie system with the

3: Using the Curie System Performing a Fluorescence Experiment You can take a fluorescence reading in either Relative Irradiance mode (I) or Scope m

3: Using the Curie System As shown in the figure, the shape of the fluorescence spectrum is impacted by the measurement mode chosen. The preferred m

3: Using the Curie System Selecting the Excitation Wavelength Range with LVF Filters To set the excitation wavelength range with the LVF filters,

3: Using the Curie System • The UV LVF filter can be used to select excitation energy in the 230 to 500 nm range. The bandwidth is fixed and varies

Table of Contents About This Manual ... iii D

3: Using the Curie System Xenon Lamp Reflected Off of Standard Mirror • Cold mirror – (Mirror Adjustment Knob turned with UV label closest to the

3: Using the Curie System In the figure below, the spectrum of the xenon lamp reflected off of the cold mirror is shown. Note that less visible lig

3: Using the Curie System Adjusting the LVFs to Select the Excitation Wavelength Range With the software in Scope mode, the CVD-DIFFUSE in the cuve

3: Using the Curie System Curie UV LVF 350 nm 640-11100-000-02-0406 27

3: Using the Curie System Curie VIS LVF 500 nm Once you have chosen your excitation wavelength range, make a note of the location on the LVF scale

3: Using the Curie System 29 If the signal you collect . . . You can . . . By . . . Has too little light Increase the light level on scale in

3: Using the Curie System Saturated Fluorescein Signal in Relative Irradiance Mode 30

3: Using the Curie System Examples of Unsaturated Signal Unsaturated Fluorescein Signal in Scope Mode 640-11100-000-02-0406

3: Using the Curie System Unsaturated Fluorescein Signal in Relative Irradiance Mode 32

Chapter 4 Troubleshooting Introduction This chapter contains the steps you need to take to solve possible problems that you may encounter with your C

Table of Contents Chapter 3: Using the Curie System...19 Introduction...

4: Troubleshooting • For Windows 2000/XP: a. From the desktop, right-click My Computer and select Properties. The System Properties screen appears

4: Troubleshooting 2. Locate Other Devices and expand the selection by clicking on the " + " sign to the immediate left. Note Improperl

4: Troubleshooting Problem 2: Older Version of OOIBase32 Installed If the computer you want to use to interface to your Curie system already has an

Appendix A Specifications Curie System Table 1: Curie System Specifications Specification Value System Dimensions 33 cm x 24.9 cm x 12.8 cm Weight 6

A: Specifications Table 1: Curie System Specifications (Cont’d) Specification Value Optical Bench and Detector Detector 2048-element linear silico

A: Specifications Table 1: Curie System Specifications (Cont’d) Specification Value Optical Bench and Detector (Cont’d) Stray Light: At 600 nm

A: Specifications Table 3: Discharge Capacitor Options Capacitor (µfd) Max Input/Flash (mJ) Max Flash Rate @600 VDC (Hz) Max Flash Rate @400 VDC

Appendix B Calibrating the Wavelength of the Curie Spectrometer Your Curie system’s spectrometer is calibrated before it leaves Ocean Optics and the

B: Calibrating the Wavelength of the Curie Spectrometer Calibrating the Wavelength of the Spectrometer Preparing for Calibration To recalibrate the

B: Calibrating the Wavelength of the Curie Spectrometer 5. Use the spreadsheet program or calculator to create a table like the one shown in the fo

About This Manual Document Purpose and Intended Audience This document provides you with an installation section to get your system up and running.

B: Calibrating the Wavelength of the Curie Spectrometer 7. Select the true wavelength as the dependent variable (Y). Select the pixel number, pixe

B: Calibrating the Wavelength of the Curie Spectrometer 7. Click the Next button. The Start Installation screen appears. 8. Click the Next button

B: Calibrating the Wavelength of the Curie Spectrometer 46 640-11100-00

Appendix C Relative Irradiance Mode Irradiance is the amount of energy at each wavelength emitted from a radiant sample. In relative terms, it is a c

C: Relative Irradiance Mode Common applications include characterizing the light output of LEDs, incandescent lamps, and other radiant energy source

C: Relative Irradiance Mode Note If you are using Microsoft Excel, choose Tools | Add-Ins and select AnalysisToolPak and AnalysisToolPak-VBA. Calibr

C: Relative Irradiance Mode 50 640-11100-000-02-0406

Appendix D Filter Sets The following table lists the specifications for the filters included with the Curie. Wavelength Information Type Center Lambd

D: Filter Sets 52 640-11100-000-02-0406

Index A application tips, 28 C calibration preparing for, 42, 48 procedure, 42, 49 saving new coefficients, 44 spectrometer, 42, 48 wavelength, 41

About This Manual You can access documentation for Ocean Optics products by visiting our website at http://www.oceanoptics.com. Select Technical → O

Index 640-1110O OOIBase32 Install, 4 operating requirements, 4 operator, 9 P PC compatibility, 40 power conn

Chapter 1 Introduction Overview The Ocean Optics Curie UV-VIS Emission Spectrofluorometer is a self-contained fluorescence system (the Curie system)

1: Introduction • Novel filtering technology. The Curie system is the only emission fluorometer with built-in linear variable filters (LVFs). These

1: Introduction Curie System Left Panel Other Items Included with Shipment Your Curie Fluorescence System from Ocean Optics should also contain the