Ocean-optics Overture User Manual

Installation Software User’s GuideDocument Number 000-20000-400-01-201108Offices: Ocean Optics, Inc. World Headquarters830 Douglas Ave., Dunedin, FL,

Chapter 1: Introduction to Spectroscopy2 000-20000-400-01-201108If you want to find out how a motorcycle works, the best thing to do is take it apart

Chapter 1: Introduction to Spectroscopy000-20000-400-01-201108 3The CCD array spectrometerLooking at sunlight through the spectrometer will tell us a

Chapter 1: Introduction to Spectroscopy4 000-20000-400-01-201108Here is what an emission spectrum looks like. This is from a Hydrogen lamp for observi

000-20000-400-01-201108 5Chapter 2Overture Software Product OverviewOverture is a spectroscopy operating software platform for 32-bit and 64-bit Windo

Chapter 2: Overture Software 6 000-20000-400-01-201108Overture InstallationOverture can be downloaded from the Ocean Optics Software Downloads site, o

000-20000-400-01-201108 7Chapter 3Overture Software Icons and MenusOverviewThe following table provides a quick guide to the Overture Software icons.

Chapter 3: Overture Software Icons and Menus8 000-20000-400-01-201108Overture Software IconsIcon FunctionNew GraphOpen SpectrumSave SpectrumCopy Data

Chapter 2: Overture Software Icons and Menus000-20000-400-01-201108 9Integration Time Integration time is the exposure time for each pixel in the arra

Chapter 3: Overture Software Icons and Menus10 000-20000-400-01-201108ColorOverture can fill the space under the spectrum with an artificial display o

Chapter 2: Overture Software Icons and Menus000-20000-400-01-201108 11AbsorbanceNoteTransmission and Absorbance modes are only enabled when dark and r

Additional Offices:Ocean Optics Asia 666 Gubei Road, Kirin Tower, Suite 601B, Changning District, Shanghai, PRC. 200336Phone 86.21.5206.8686Fax 86.2

Chapter 3: Overture Software Icons and Menus12 000-20000-400-01-201108For absorbance measurements, high resolution is not required, so smoothing makes

Chapter 2: Overture Software Icons and Menus000-20000-400-01-201108 13Zoom Zoom In lifts the highest peak to the full y axis scale. Zoom Out cancels t

Chapter 3: Overture Software Icons and Menus14 000-20000-400-01-201108CursorClick anywhere on the graph to launch the cross hairs cursor. The cursor x

Chapter 2: Overture Software Icons and Menus000-20000-400-01-201108 15Reference Lines Emission lines are referenced using the line spectra library. St

Chapter 3: Overture Software Icons and Menus16 000-20000-400-01-201108The reference lines are sharp and the real emission lines appear to be not so sh

Chapter 2: Overture Software Icons and Menus000-20000-400-01-201108 17Menu FunctionsThis section details the various functions available from the Over

Chapter 3: Overture Software Icons and Menus18 000-20000-400-01-201108OpenThis menu item allows you to choose whether to show data in a new window or

Chapter 2: Overture Software Icons and Menus000-20000-400-01-201108 19ExitThis menu selection allows you shut down Overture.NewThis menu item has one

Chapter 3: Overture Software Icons and Menus20 000-20000-400-01-201108

000-20000-400-01-201108 21Chapter 4ExperimentsConcentration wizardThe concentration wizard guides you through the process of measuring absorbance at d

000-20000-400-01-201108 iTable of ContentsAbout This Manual...

Chapter 4: Experiments22 000-20000-400-01-201108NoteIf you change the integration time or anything else in the experiment setup, you will need to stor

Chapter 4: Experiments000-20000-400-01-201108 234. Choose Beer-Lambert Law or Calibration. To use the Beer-Lambert Law option you need to know the mol

Chapter 4: Experiments24 000-20000-400-01-2011086. Create the calibration curve. Take at least three samples of known concentration. Either enter the

Chapter 4: Experiments000-20000-400-01-201108 25FluorescenceThe sampling unit can be set up to measure fluorescence from a side port at 90 degrees to

Chapter 4: Experiments26 000-20000-400-01-201108In transmission mode the same absorbance (now a dip) is seen. We know that light energy is being absor

Chapter 4: Experiments000-20000-400-01-201108 27The spectrum below is taken from the side port using the fluorescence fiber. It shows a peak at 520 nm

Chapter 4: Experiments28 000-20000-400-01-201108

000-20000-400-01-201108 29Chapter 5ApplicationsPhysics applicationsThe sunThe spectrometer can resolve the strongest Fraunhofer lines. These can be se

Chapter 5 Applications30 000-20000-400-01-201108Most of these lines originate from the sun’s chromosphere, but the strong 761nm absorption dip is caus

Chapter 5 Applications000-20000-400-01-201108 31Reflection of light from colored surfacesThe spectral emission of scattered light from surfaces shows

Table of Contentsii 000-20000-400-01-201108Copy Data to Clipboard ...

Chapter 5 Applications32 000-20000-400-01-201108Hydrogen spectrum Balmer linesCalculation of the Rydberg constant from the Balmer series lines. Match

Chapter 5 Applications000-20000-400-01-201108 33Standard flame test compounds can be used to show how all the metallic elements produce line spectra.

Chapter 5 Applications34 000-20000-400-01-201108Chemistry applicationsFollowing are some of the color-related experiments that can be carried out with

Chapter 5 Applications000-20000-400-01-201108 35Spectrophotometric analysis of commercial aspirinAcetyl Salicylic Acid (ASA) complex ion is formed by

Chapter 5 Applications36 000-20000-400-01-201108

000-20000-400-01-201108 37Appendix ASample ExperimentsBeer’s law analysis of KMnO4Equipment Needed: Ocean Optics CCD array spectrometer with lamp/cuv

Appendix A: Sample Experiments38 000-20000-400-01-2011086. In the Concentration Wizard, select “Calibrate from solutions of known concentration”.7. In

Appendix A: Sample Experiments000-20000-400-01-201108 39 Flasks, beakers and pipettes for preparationObjectives:Olive oils contain varying amounts of

Appendix A: Sample Experiments40 000-20000-400-01-201108 Stand and clamp  Flame test wire loop  Banana or dried banana chips  Salts: LiCl, NaCl, S

Appendix A: Sample Experiments000-20000-400-01-201108 41Spectrophototmetric analysis of a buffer solution – Isobestic pointProcedure:1. Measure and an

Table of Contents000-20000-400-01-201108 iiiWater quality testing using indicator reagents...

Appendix A: Sample Experiments42 000-20000-400-01-201108Fluorescence and Stokes shiftObjectives:Using the side port of the sampling unit the Stokes sh

000-20000-400-01-201108 43 Appendix BMaintenanceFiber careThe fiber optic cables have a glass core. They should not be bent with a radius of less tha

Appendix B: Maintenance44 000-20000-400-01-201108TroubleshootingProblem Possible Cause(s) Suggested Solution(s)The icons are grayed-out USB cable is n

Appendix B: Maintenance000-20000-400-01-201108 45Can I use the spectrometer outside?Yes, but only in dry, warm conditions. Do not expose the case to d

Appendix B: Maintenance46 000-20000-400-01-201108

000-20000-400-01-201108 47IndexAabsorbancemode, 11number, 34applications, 29chemistry, 34physics, 29average scans, 11BBalmer lines, 32Beers Law, 34Bee

48 000-20000-400-01-201108Iicons, 8installation, 5via CD, 6via download, 6integration time, 9intensity mode, 9ionized gases, 31isosbestic, 34Llasers,

Table of Contentsiv 000-20000-400-01-201108

000-20000-400-01-201108 vAbout This ManualDocument Purpose and Intended AudienceThis document provides you with installation and operation instruction

About This Manualvi 000-20000-400-01-201108You can also access operating instructions for Ocean Optics products on the Software and Technical Resource

000-20000-400-01-201108 1Chapter 1Introduction to SpectroscopySpectroscopy in a nutshellA spectrometer is a device that breaks up light into different