Ocean-optics CHEM2000 User Manual

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OOICHEM Software
User's Guide
Document Number 105-00000-CHM-02-0405
PRELIMINARY DRAFT
Offices: Ocean Optics, Inc.
830 Douglas Ave., Dunedin, FL, USA 34698
Phone (727) 733-2447
Fax (727) 733-3962
8:30 a.m.–6 p.m. EST
Ocean Optics B.V. (Europe)
Nieuwgraaf 108 G, 6921 RK DUIVEN, The Netherlands
Phone 31-(0)26-3190500
Fax 31-(0)26-3190505
E-mail: [email protected] (General sales inquiries)
[email protected] (European sales inquiries)
[email protected] (Questions about orders)
[email protected] (Technical support)
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Summary of Contents

Page 1 - User's Guide

OOICHEM Software User's Guide Document Number 105-00000-CHM-02-0405 PRELIMINARY DRAFT Offices: Ocean Optics, Inc. 830 Douglas A

Page 2 - Limit of Liability

1: Introduction • Color Temperature. Enter the color temperature of your reference light source used in relative irradiance measurements. Acquisiti

Page 3 - Table of Contents

Chapter 2 Using OOIChem Software OOIChem software provides users with five different modes of operation: Scope, Absorbance, Transmission, Relative

Page 4

2: Using OOIChem Software Display Functions Several functions are accessed not through the menu but through buttons and task bars directly on the di

Page 5 - About This Manual

2: Using OOIChem Software Transmission Selecting this mode switches the spectral window into Transmission Mode. This is also the spectral processin

Page 6 - Upgrades

2: Using OOIChem Software The absorbance of a solution is related to the concentration of the species within it. The relationship, known as Beer’s L

Page 7 - Introduction

2: Using OOIChem Software Cursor Properties In this toolbar, you can label the cursor and monitor the cursor’s X value and Y value. To the right of

Page 8 - Quick Start

2: Using OOIChem Software blank in the sampling region. Storing a reference spectrum is requisite before the software can calculate absorbance, tran

Page 9 - Spectrometer Configuration

2: Using OOIChem Software Scaling the Graph You can change the vertical and/or horizontal scales of the graph by simply clicking on an X and Y endp

Page 10 - Step 3: Receive Data

2: Using OOIChem Software Exit Select File | Exit from the menu to quit OOIChem. A message box appears asking you if you are sure you want to exit t

Page 11 - Using OOIChem Software

2: Using OOIChem Software Show Legends Select Edit | Show Legends to enable or disable the legends for the spectral trace, overlays, and kinetics t

Page 12 - Display Functions

Copyright © 2000-2005 Ocean Optics, Inc. All rights reserved. No part of this publication may be reproduced, sto

Page 13 - Concentration

2: Using OOIChem Software Enable Strobe This function allows you to enable or disable the triggering of external strobes through the spectrometer.

Page 14 - Cursor Function Bar

2: Using OOIChem Software 2. Supply a line from Pin 8 of the J2 Accessory Connector to your triggering device. (See figure for pin location.) 3.

Page 15 - Reference Scan

2: Using OOIChem Software Preset Duration Enter a value to set the length of time for the entire kinetics process. Be sure to select hours, minutes

Page 16 - Acquire Data Modes

Chapter 3 Experiment Tutorial Overview When you are ready to begin your experiment, you should have already installed the Maxwell and OOIChem softw

Page 17 - File Menu Functions

3: Experiment Tutorial ► Procedure To take an absorbance measurement: 1. Select Scope under Mode of Operation in the software display area. Make su

Page 18 - Edit Menu Functions

3: Experiment Tutorial The software calculates %Tλ (or %Rλ) by the following equation: Sλ - Dλ%Tλ= Rλ - Dλx 100%Where: S = Sample intensity at wav

Page 19 - Spectrometer Menu Functions

3: Experiment Tutorial Note If at any time any sampling variable changes – including integration period, averaging, boxcar smoothing, distance from

Page 20 - Enable Strobe

3: Experiment Tutorial 2. Select Scope under Mode of Operation in the software display area. Make sure the signal is on scale by adjusting acquisiti

Page 21 - Kinetics Configuration

3: Experiment Tutorial To discover the unknown concentration of a substance in a solution, you must first take spectral scans of a series of soluti

Page 22 - Mathematical Calculations

3: Experiment Tutorial 8. Click the Scan button or select Scan | Solution from the menu. The absorbance value will appear next to the concentration

Page 23 - Experiment Tutorial

Table of Contents About This Manual ... iii D

Page 24 - Transmission Experiments

3: Experiment Tutorial 14. Select Edit | Show Legend from the menu to display the legend for the calibration curve. The legend allows you to choose

Page 25

3: Experiment Tutorial 2. Select Spectrometer | Kinetics Configuration from the menu to open the Kinetics Configuration dialog box. 3. Enter a Prese

Page 26

3: Experiment Tutorial 26 PRELIMINARY DRAFT 105-00000-CHM-02-0405

Page 27 - Concentration Experiments

PRELIMINARY DRAFT 27 Index A absorbance experiment, 17 Absorbance mode, 6 Acquire Data modes, 10 Chart Active, 10 Acquisition parameters, 9 Averag

Page 28 - 3: Experiment Tutorial

Index PRELIMINARY DRAFT 105-00000-CHM-02-0405 F File Menu functions, 11 Exit, 12 Open Kinetics Values, 11 Open Spectral Overlay, 11 Print Spectra an

Page 29 - 3: Experiment Tutorial

Index PRELIMINARY DRAFT 29 S scaling the graph, 11 scan dark, 10 reference, 9 Scan, 13 Scan/Stop button, 10 Scope mode, 6 Select Concentration Wavel

Page 30 - Kinetics Experiments

Index PRELIMINARY DRAFT 105-00000-CHM-02-0405 30

Page 31

Table of Contents File Menu Functions ... 11Sav

Page 32

About This Manual Document Purpose and Intended Audience This document provides information for installing and operating OOIChem software with the Ma

Page 33 - PRELIMINARY DRAFT 27

About This Manual Upgrades Occasionally, you may find that you need Ocean Optics to make a change or an upgrade to your system. To facilitate these

Page 34 - Index

Chapter 1 Introduction Company History Ocean Optics miniature fiber optic spectrometers and accessories have revolutionized the analytical instrume

Page 35 - PRELIMINARY DRAFT 29

1: Introduction OOIBase32 Spectrometer Operating Software OOIBase32 is our standard spectrometer operating software that we provide free of charge t

Page 36

1: Introduction 5. Select a Program Manager Group. Click Next. At the Start Installation dialog box, click Next. 6. At the Installation Complete d

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